Phytochemical Investigation of Egyptian Spinach Leaves, a Potential Source for Antileukemic Metabolites: In Vitro and In Silico Study

Abstract Spinacia oleracea L., Amaranthaceae, leaves cultivated in Egypt demonstrated a potential antileukemic activity against the chronic myeloid leukemia, K562 cell line. Thus, aim of this study is to carry out phytochemical investigation S. as well isolation its phytoconstituents. Phytochemical resulted seventeen known compounds. The biological revealed that compounds hexaprenol, phytol, and 18-[(1-oxohexadecyl) oxy]-9-octadecenoic acid exhibited remarkable antiproliferative cells vitro . A mechanistic silico showed strong binding affinity towards topoisomerase (docking score −12.50, −9.19, −13.29 kcal/mol, respectively), Abl kinase −11.91, −9.35, −12.59 respectively). Molecular dynamics produced stable complexes with both RMSD values 1.81 1.85 Å, respectively. As result our findings, we recommend more vivo preclinical studies confirm benefit spinach for leukemia patients. Graphical